Bioprocess Development

Veeda Biopharma’s Bioprocess Development team specializes in the development of therapeutic and non-therapeutic proteins using microbial (E. coli) and mammalian (CHO; HEK) expression systems. We focus on creating high-yield, stable clones with comprehensive traceability throughout the process. Starting at the microbioreactor level, we scale up production in 5 L bioreactors with the capability to expand to a 50 L single-use bioreactor, strictly adhering to GMP and GLP standards.

A scientist in a cleanroom with complex bioprocess development equipment, showcasing Veeda's advanced lab capabilities.

Precision in Every Step:
Advanced Process Development Solutions

Diagram of Cell Line Development for bioprocess applications, showing microbial and viral cell line options.
Diagram illustrating bioprocess Process Development services, showing icons for engineering and biological applications.

Your Partner for Mammalian Protein Expression
& Cell Line Development

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Stable Pool Creation

  • Gene synthesis [codon optimization]
  • Expression construct preparation
  • Lipofection/electroporation
  • Stable pool generation
  • Stable pool evaluation

Stable pool evaluation

  • Single cell dispensing
  • Single cell imaging
  • Monoclonality establishment
  • Titer evaluation

RCB Characterization

  • Purity
  • Sterility
  • Stability

Banking of top clones

  • Evaluation of top/lead clones
  • Growth and expansion
  • Banking (RCB)

Microbial Protein Production and
Clone Development

Protein purification and
characterization

Cloning and expression of recombinant
heterologous proteins

Host cell
characterization

Integrated Expertise: Analytical CMC in Every Step of Cell line and Process Development

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Cell Line/Clone Selection

  • Protein sequence verification
  • Sequence variants characterization
  • Post-translational modification(PTM) analysis
  • Glycosylation analysis
  • De novo sequencing

Upstream

  • Harvest Titers
  • Identity
  • Purity
  • Glycosylation analysis
  • Post-Translational Modification Analysis

Downstream

  • Size and charge variants
  • Refolding efficiency
  • Identity
  • Purity
  • Heterogeneity
  • Impurity characterization
  • N-/O-glycans
  • Residuals
  • Potency

Mastering Every Stage:
End-to-End Process Development Expertise

A small-scale lab bioreactor used for bioprocess development and optimization studies.

Process Development & Optimization (100-150ML)

  • Bioreactor suitability
  • Media/feed screening
  • Feeding strategy
  • Process parameters
A benchtop bioreactor system with two vessels, representing advanced lab equipment for bioprocess development services.

Process Scale-up at 2L/5L Scalevest

  • Feeding strategies
  • Aeration strategies
  • pH
  • Material generation
A stainless steel 4-way valve used for the Harvest Clarification step in bioprocess development.

Harvest Clarification

  • Centrifugation
  • Filtration
A lab-scale chromatography system used for downstream process purification and bioprocess development.

Downstream Process

  • Affinity chromatography
  • Ion-exchange chromatography
  • Size exclusion chromatography
  • Hydrophobic interaction chromatography
  • Filtration
  • Concentration